维生素C能够治疗尿路感染-酸化尿液,把亚硝酸盐还原为杀菌的一氧化氮

Effects of pH, Nitrite, and Ascorbic Acid on Nonenzymatic Nitric Oxide Generation and Bacterial Growth in Urine

 

翻译:蓝山

 

pH、亚硝酸盐、抗坏血酸对尿中一氧化氮和细菌生长的影响

 

 

亚硝酸盐可能是由尿路感染中的细菌产生的。亚硝酸盐的酸化会导致一氧化氮(NO)和其他活性氮氧化物的形成,这对各种微生物都是有毒的。我们研究了含亚硝酸盐和抗坏血酸、轻度酸化的尿液中一氧化氮的形成和细菌生长。

 

从健康的受试者中收集的维生素c .尿液在不同pH值的封闭注射器中孵育,并添加了不同数量的亚硝酸盐和/或抗坏血酸。使用化学发光技术来测量头空间气体的生成。类似的设置也用于研究尿液中三种细菌的生长。轻度酸化的含硝酸盐的尿液会产生大量的NO,这一生产被抗坏血酸极大地增强了。在酸化尿液中加入亚硝酸盐后,大肠杆菌、铜绿假单胞菌和葡萄球菌的生长明显减少。抗坏血酸增强了这种抑制作用。综上所述,当亚硝酸盐存在时,我们发现三种常见的尿病原菌在轻度酸化的尿液中有明显的抑制作用。酸化亚硝酸盐的抑菌作用可能与NO和其他有毒活性氮中间体的释放有关。这些结果可能有助于解释尿酸化的众所周知的有益影响,例如,维生素C在治疗和预防尿路感染.

 

 

Effects of pH, Nitrite, and Ascorbic Acid on Nonenzymatic Nitric Oxide Generation and Bacterial Growth in Urine

 

https://doi.org/10.1006/niox.2001.0371Get rights and content

 

Abstract

Nitrite may be generated by bacteria in urine during urinary tract infections. Acidification of nitrite results in the formation of nitric oxide (NO) and other reactive nitrogen oxides, which are toxic to a variety of microorganisms. We have studied NO formation and bacterial growth in mildly acidified human urine containing nitrite and the reducing agent vitamin C. Urine collected from healthy subjects was incubated in closed syringes at different pH values with varying amounts of nitrite and/or ascorbic acid added. NO generation was measured in headspace gas using a chemiluminescence technique. A similar setup was also used to study the growth of three strains of bacteria in urine. Mildly acidified nitrite-containing urine generated large amounts of NO and this production was greatly potentiated by ascorbic acid. The growth of Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus saprophyticus was markedly reduced by the addition of nitrite to acidified urine. This inhibition was enhanced by ascorbic acid. In conclusion, we show that the growth of three common urinary pathogens is markedly inhibited in mildly acidified urine when nitrite is present. The bacteriostatic effect of acidified nitrite is likely related to the release of NO and other toxic reactive nitrogen intermediates. These results may help to explain the well-known beneficial effects of urinary acidification with, e.g., vitamin C in treatment and prevention of urinary tract infection.

https://www.sciencedirect.com/science/article/pii/S1089860301903714