人卵巢癌细胞的自裂: 扫描电子和光镜下,维生素C和维生素K3治疗诱导的一种新的细胞死亡

Autoschizis of human ovarian carcinoma cells: Scanning electron and light microscopy of a new cell death induced by sodium ascorbate: Menadione treatment

 

翻译:蓝山

 

用抗坏血酸钠 (VC)、维生素K3 (甲萘醌,VK3)、或两者按100:1比例的组合处理人卵巢癌(MDAH 2774)细胞,然后用扫描电子显微镜(SEM)和光学显微镜(LM)进行检查。光学显微镜的数据证实了SEM的观察结果,证明VC-VK3治疗的肿瘤细胞的死亡主要是由自体分裂引起的。这种类型的细胞死亡的特征是细胞体积缩小、细胞质自我切除、细胞核和核仁形态学降解,而不形成凋亡体。最终,细胞死亡是由核分裂和核溶解引起的。

 

本研究表明,在VC治疗导致细胞浆膜损伤(分支丝状伪足、水泡、气泡); 细胞骨架损伤和自噬是由VCVK3VK +VK引起的。VC处理导致细胞直径减少23%VK3处理细胞的细胞直径减少66%。在VK3治疗1h后,发现一种异源细胞。这一种群可以被分解成一个种群,其直径比含SHAM处理的细胞的直径小23%,另一个种群的直径大约是经过处理的细胞的两倍。第二种群显示成对物的形成,看来细胞在分裂(自体分裂细胞死亡的早期)。其中的一半含有细胞核,另一半由细胞质和膜组成。这个双重核的核部分将被切除。在VK3治疗后,经点算细胞死亡的类型,43%的细胞死于自体分裂,3%由凋亡,1.9%由肿瘤坏死。这些结果证实,自体分裂是细胞死亡的主要形式,是体外用维生素组合治疗人类卵巢癌细胞的结果。

 

Autoschizis of human ovarian carcinoma cells: Scanning electron and light microscopy of a new cell death induced by sodium ascorbate: Menadione treatment

 

Abstract

Human ovarian carcinoma (MDAH 2774) cells were treated with sodium ascorbate (VC), menadione (VK3), or a combination of both in a ratio 100:1 for 1h and then examined with scanning electron microscopy (SEM) and light microscopy (LM). Light microscopy data corroborated SEM observations, which demonstrated that death of VC+VK3-treated tumor cells occurred primarily by autoschizis.

 

This type of cell death is characterized by a decrease in cell size, cytoplasmic self-excisions, and nuclear and nucleolar morphologic degradations without the formation of apoptotic bodies. Ultimately, cell death results from karyorrhexis and karyolysis.

 

This study illustrates that plasma membrane damage (branching filopodia, blisters, blebs) results from VC treatment; cytoskeletal damage and self-morsellation are caused by VC, VK3 and VC+VK, treatments. The VC treatment results in a 23% decrease in cell diameter while VK3-treated cells decrease cell diameter by 66%. After 1h of VC+VK3 treatment,

 

a heterogenous cell population is found. This population can be resolved into one population whose diameters are 23% smaller than those of sham-treated cells, and a second population whose diameters are approximately twice those of sham-treated cells. This second population is indicative of doublet formation in which the cells appear to be dividing (an early stage of autoschizic cell death). One half of the doublet contains the cell nucleus while the other half consists of cytoplasm and membrane only. The enucleate portion of this doublet will then be excised. When the types of cell death are enumerated following VC+VK3 treatment, 43% of the cells die by autoschizis, 3% by apoptosis, and 1.9% by oncosis. These results confirm that autoschizis is the principal form of cell death that results from the in vitro treatment of human ovarian carcinoma cells with the vitamin combination.

 

Autoschizis of human ovarian carcinoma cells: Scanning electron and light... | Request PDF. Available from: https://www.researchgate.net/publication/10656714_Autoschizis_of_human_ovarian_carcinoma_cells_Scanning_electron_and_light_microscopy_of_a_new_cell_death_induced_by_sodium_ascorbate_Menadione_treatment [accessed Feb 19 2018].